The five of them called themselves Go-ninja when attacking Toyama Chintarou's organization (The one who they believed had kidnapped Elizabeth). So she trained Gintoki, Katsura, Shinpachi and Kagura to be one day ninja. Gintoki asked her to help them saving Elizabeth because her ability as a kunoichi. As she's very often lurking in Gintoki's house or following him around, she took part in many events the Yorozuya experienced. She also spends her time fighting with Tae, who she considers her love-rival. When she is not working she seems to be spending most of her time stalking Sakata Gintoki.
Sarutobi Ayame is an assassin, but she also has a part-time job in the Kunoichi Kafé, where she works as a waitress.
She wears black boots and her legs are mostly uncovered, except for some black hot-pants. Her arms are covered by dark purple forearm sleeves that matches her scarf in the same color, and on her left breast she has a protection cup. Her usual attire consists of a Martial Arts training uniform top with its arms cut off and a red band wrapped around the waist. There is a beauty mark under her right eye, and she is apparently big-breasted (mentioned by both Gintoki and Kagura). She has long, light purple hair and wears dark-red glasses. She attended the funeral of Zenzou's Father and it came to light that both she and Wakikaoru were his students and attended the same class together, during which they would often play Kick-the-can because it demanded the ability for them to hide themselves and make instantaneous decisions. She was trained as a ninja in the Oniwabanshuu, but would later resign and become an assassin, as seen written in her resumé. 10.1 Gintama The Final Chapter: Be Forever Yorozuya.